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KMID : 0385219910010020131
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Korean Journal of Gerontology
1991 Volume.1 No. 2 p.131 ~ p.141
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Comparison of the Effects of 1-Methyl-4-Phenyl-1,2,3,6,-Tetrahydropyridine(MPTP), 1-Methyl-4-Phenylpyridine(MPP+) and Paraquat on Lipid Perxidation and Deoxyribose Degradation
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Cheong Myong-Hee
Myung Ho-Jin
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Abstract
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1-Methyl-4-Phenylpyridine(MPP+) is the putative toxic metabolite of 1-Methyl-4-Phenyl-1,2,3,6,-Tetrahydropyridine(MPTP) and is structurally similar to the herbicide paraquat(PQ) which is an active redox-cycling agent for the formation of reactive oxygen metabolites. Therefore, to evaluate the involvement of reactive oxygen metabolites in MPTP toxicity, MPP+ and MPTP were compared with PQ with respect to their ability to produce lipid peroxidation and deoxyribose degradation induced by enzymatic and nonenzymatic systems as parameters of cellular damage in vitro. In this present study, PQ stimulated lipid peroxidation and deoxyribose degradation in enzymatic system, but failed to affect this two parameters in nonenzymatic system. But MPP+ inhibited the degradation od deoxyribose in both reaction system and PQ-stimulated lipid peroxidation in enzymatic system. MPTP inhibited this two parameters in both reaction systems and its inhibitory effect was more powerful in nonenzymatic system. Moreover, only MPTP catalyzed the autooxidation of reduced iron. From these results, MPP+ and MPTP did not act like PQ to stimulate lipid peroxidation and deoxyribose degradation. In addition, MPP+ and MPTP showed antioxidant effects in these reaction conditions, but MPP+ is less potent than MPTP to exert antioxidant effects. Thus, it can be expected that the generation of reactive oxygen metabolites like the toxic mechanism of PQ is unlikely to be involved in MPTP and MPP+ toxicity in vitro. While interaction of Fe2+ with MPTP may be related its antioxidant effects, it is suggested that another mechanism is involved in these antioxidant actions of MPTP and MPP+
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KEYWORD
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1-Methyl-4-Phenyl-1, 2, 3, 6-Tetrahydropyridine(MPTP), 1-Methyl-4-phenylpyridine(MPP +), Paraquat, Redox-cycling, Reactive oxygen metabolites
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